Biology

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Abstract. Yeast is distributed to bakeries, breweries and local stores in large quantities. Two cultures were evaluated in an attempt to figure out which one was contaminated with antibiotics. Both cultures were tested with the use of a hemocytometer. Which culture was Infected with the antibiotics did not make a difference because antibiotics attack bacteria, and yeast is not bacteria.

Introduction

The usage of a clean hemocytometer was mandatory in order to obtain accurate and legitimate readings and counts of the cells. The hemocytometer's chamber was filled with the yeast from Culture A and then the process was repeated with the yeast from Culture B. The culture had to be drawn from the beaker with extreme caution, for if the yeast cells that were extracted were homogenous or else the results of the experiment would be inaccurate. Capillary action draws the contents from the pipette into the hemocytometer's chamber and so the liquid is not spill out and onto the slide. The fact that no liquid spilled onto the slide was important because if such happened the cell count of the hemocytometer would be tainted.

Culture A

Figure 1

The numbers in this diagram show the cell count that was obtained from Culture A. The numbers that are shown on the outside of the main central four quadrants are the numbers that pertain to the cell count of culture A. It is noticeable that the numbers seen here are higher than the numbers for the cell count of culture B. An assumption can be made that culture A was no the culture infected with antibiotics because the cell count for this culture is higher. If this culture was the one that had been infected there should have been a yield of cells and the number in the cell count should have dropped. In order to prove this hypothesis the total number of cells for culture B must be accumulated and a conclusion must be reached.

Culture B

Figure

The numbers seen in this figure are the total number of cells that were counted with the use of the hemocytometer and the microscope. Here the difference between the cell count of culture A and culture B becomes more obvious. It can be concluded that the cell count for Culture A was much higher than that of Culture B. Now it has become easy to identify that Culture B was in fact the culture that was contaminated with the antibiotics. This culture shows that the number of cells have yielded, and therefore, the total number is much more lower.

Conclusion

The initial hypothesis was that the contamination of an unspecified culture with antibiotics would not affect the cell count of the yeast. After extensive experimentation this initial hypothesis was proven to be wrong. Antibiotics do indeed cause a reaction in Culture B.

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